Plasmid_Backbone

Part:BBa_K797013:Design

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-21)

Plasmid Backbone for golden gate assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2197
    Illegal suffix found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 16
    Illegal PstI site found at 30
    Illegal NotI site found at 23
    Illegal NotI site found at 2203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2197
    Illegal XhoI site found at 1047
    Illegal XhoI site found at 2073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2197
    Illegal suffix found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2197
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2212
    Illegal SpeI site found at 16
    Illegal PstI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 2224


Design Notes

The terminal of this part was modified to use it for Golden gate assembly


Source

This part was made by PCR　from iGEM part BBa_PSB1K3

References

[1]Carola Engler, Romy Kandzia, Sylvestre Marillonnet "A One Pot, One Step, Precision Cloning Method with High Throughput Capability"PLoS ONE 3(11): e3647. [2]Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet"Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes" PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553 doi:10.1371/journal.pone.0003647